MTMDAT-HADDOCK: High-throughput, protein complex structure modeling based on limited proteolysis and mass spectrometry
نویسندگان
چکیده
منابع مشابه
High-throughput affinity mass spectrometry.
Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biologica...
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Limited proteolysis experiments can be successfully used to probe conformational features of proteins. In a number of studies it has been demonstrated that the sites of limited proteolysis along the polypeptide chain of a protein are characterized by enhanced backbone flexibility, implying that proteolytic probes can pinpoint the sites of local unfolding in a protein chain. Limited proteolysis ...
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Proteomics as it is most commonly referred to is the study of the total complement of proteins in a closed system, the protein complement of the genome. Proteomics is a new kind of systems biology. Instead of studying one or more components of a system at a time, proteomics strives to study the whole system at once. Proteomics strives to do for proteins, what microarrays do for nucleic acids. P...
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The success of mass spectrometry based proteomics depends on efficient methods for data analysis. These methods require a detailed understanding of the information value of the data. Here, we describe how the information value can be elucidated by performing simulations using synthetic data.
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With the accumulation of next generation sequencing data, there is increasing interest in the study of intra-species difference in molecular biology, especially in relation to disease analysis. Furthermore, the dynamics of the protein is being identified as a critical factor in its function. Although accuracy of protein structure prediction methods is high, provided there are structural templat...
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ژورنال
عنوان ژورنال: BMC Structural Biology
سال: 2012
ISSN: 1472-6807
DOI: 10.1186/1472-6807-12-29